The present investigation deals with the floristic study of 34 taxa of Chlorophyceae belonging to 16 genera from Kodamdesar and Kalyan Sagar ponds of Bikaner region. Present investigation was carried out for the period of one year from July,2012 to June,2013. The maximum species were noted of Scenedesmaceae and Desmidiaceae (9) followed by Hydrodictyaceae (6), Selenastraceae (4), Coelastraceae (2) Cholorococcaceae (1), Botryococcaceae (1), Characiaceae (1) and Zygnemaceae (1). Scenedesmus were designated as major taxa showing maximum variation in species diversity.